The Government says the fuel stockpile will help producers make planting and operational decisions with greater confidence, while also supporting food security and helping to ease pressure on grocery prices.
The reserve will not affect normal day-to-day fuel supply and will only be accessed in the event that Level 4 of the National Fuel Security Plan is activated. It will work alongside the Commonwealth's Minimum Stockholding Obligation for fuel companies.
Authorities have confirmed there remains sufficient diesel supply across Victoria, with regular shipments continuing to arrive as required.
Premier Jacinta Allan said strong harvests depend on reliable fuel access for regional communities.
"Backing farmers means backing local jobs and businesses and helping keep grocery prices down for everyone," she said.