Despite her legal woes, Santanchè survived a no-confidence motion in 2023 and had long enjoyed Meloni's support.
Santanchè announced her resignation in a statement to Meloni, in which she said she would "obey" Meloni's wishes and acknowledged an abrupt interaction on Tuesday following the prime minister's resignation demand. She said she did not want to be made "a scapegoat" for the referendum defeat, underlining that the measure passed in her northern region of Lombardy and district.
"I won't hide from you a degree of bitterness over how my ministerial journey has ended, but in my life I am accustomed to paying my own debts - and often those of others," Santanchè wrote.
The judicial reforms had been billed by Meloni's coalition as a key step toward streamlining Italy's judicial system, which has been criticized as being slow, bureaucratic and vulnerable to political influence.
But critics argued that the proposed measures risked concentrating too much power in the executive branch. Opposition parties, civil society groups and legal associations mounted a unified front, warning that the reform could undermine institutional checks and balances.